The 2000-01 Scottish Third Division was won by Hamilton Academical who, along with second placed Cowdenbeath, gained promotion to the Second Division. Elgin City finished bottom.
| P | Team | Pld | W | D | L | GF | GA | GD | Pts | Promotion |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Hamilton Academical | 36 | 22 | 10 | 4 | 75 | 41 | 34 | 76 | Promoted to Second Division 2001-02 |
| 2 | Cowdenbeath | 36 | 23 | 7 | 6 | 58 | 31 | 27 | 76 | |
| 3 | Brechin City | 36 | 22 | 6 | 8 | 71 | 36 | 27 | 72 | |
| 4 | East Fife | 36 | 15 | 8 | 13 | 49 | 46 | 3 | 53 | |
| 5 | Peterhead | 36 | 13 | 10 | 13 | 46 | 46 | 0 | 49 | |
| 6 | Dumbarton | 36 | 13 | 6 | 17 | 46 | 49 | -3 | 45 | |
| 7 | Albion Rovers | 36 | 12 | 9 | 15 | 38 | 43 | -5 | 45 | |
| 8 | East Stirlingshire | 36 | 10 | 7 | 19 | 37 | 69 | -32 | 37 | |
| 9 | Montrose | 36 | 6 | 8 | 22 | 31 | 65 | -34 | 26 | |
| 10 | Elgin City | 36 | 5 | 7 | 24 | 29 | 65 | -36 | 22 |
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